Resistance gene cloning from a wild crop relative by sequence capture and association genetics
نویسندگان
چکیده
منابع مشابه
Cloning and Sequence Analysis of Gene Encoding OipA from Iranian Clinical Helicobacter pylori
Background: Outer inflammatory protein A (OipA) is one of the important adhesins of H. pylori and a valuable candidate for vaccine development. Its gene is under "on-off" switch status which correlates with OipA protein expression. Objectives: We aimed to obtain a recombinant OipA clone (with "on" status) from an Iranian clinical isolate. Materials and Methods: A clinical H. pylori-isolate demo...
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Background: Many plant growth-promoting bacteria including Rhizobia contain the 1-aminocyclopropane-1-carboxylate (ACC) deaminase enzyme that can leave ACC, and thereby lower the level of ethylene in stressed plants. Drought and salinity are the most common environmental stress factors for plants in Iran. Objectives: The main aim of this research was development of bio-fertilizers containing A...
متن کاملAssociation Genetics and More from Crop Transcriptome Sequences
Genetic studies often exploit the capability to segregate mapping populations derived from bi-parental crosses. However, results can be obtained more quickly and with greater genetic resolution by exploiting the historical recombination between loci in genetic diversity panels. Where recombination between loci is observed rarely, those loci are said to be in Linkage Disequilibrium (LD). The ide...
متن کاملcloning and nucleotide sequence of a lipase gene from a soil isolate
the present study was aimed to isolate and characterize the lipolytic enzyme producing bacteria from soil samples of regions around zayande-rood river of isfahan, iran. soil samples were collected from 15 cm depth of soil surface. based on morphology, distinct colonies were isolated and purified through streak culture on to standard agar plates. isolated colonies were examined for lipase activi...
متن کاملCloning, expression in Escherichia coli and nucleotide sequence of a tetracycline-resistance gene from Streptomyces rimosus.
Determinants of tetracycline resistance have been cloned from two different tetracycline-producing industrial strains of Streptomyces into Streptomyces lividans using the plasmid vector pUT206. Three plasmids, pUT250 and pUT260 with a 9.5 and a 7.5 kb insert respectively of Streptomyces rimosus DNA, and pUT270 with a 14.0 kb insert of Streptomyces aureofaciens DNA, conferring resistance to tetr...
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ژورنال
عنوان ژورنال: Nature Biotechnology
سال: 2019
ISSN: 1087-0156,1546-1696
DOI: 10.1038/s41587-018-0007-9